Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.

Swarts, Daan C; Jinek, Martin

Swarts, Daan C; Jinek, Martin.

Affiliation

Swarts DC; Department of Biochemistry, University of Zurich, 8057 Zurich, Switzerland.
Jinek M; Department of Biochemistry, University of Zurich, 8057 Zurich, Switzerland. Electronic address: jinek@bioc.uzh.ch.

Mol Cell ; 73(3): 589-600.e4, 2019 02 07.

Article in En | MEDLINE | ID: mdl-30639240

Subject(s)

Bacterial Proteins/metabolism; CRISPR-Associated Proteins/metabolism; CRISPR-Cas Systems; DNA, Single-Stranded/metabolism; Francisella/enzymology; Gene Editing/methods; RNA, Guide, Kinetoplastida/metabolism; Bacterial Proteins/genetics; CRISPR-Associated Proteins/chemistry; CRISPR-Associated Proteins/genetics; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/genetics; Enzyme Activation; Francisella/genetics; Models, Molecular; Nucleic Acid Conformation; Protein Conformation; RNA, Guide, Kinetoplastida/chemistry; RNA, Guide, Kinetoplastida/genetics; Structure-Activity Relationship; Substrate Specificity

Key words

CRISPR-Cas; Cas12a; Cas9; Cpf1; DNA cleavage; DNase; PAM; genetic engineering; genome editing; ssDNase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / DNA, Single-Stranded / RNA, Guide, Kinetoplastida / CRISPR-Associated Proteins / CRISPR-Cas Systems / Gene Editing / Francisella Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article Affiliation country: Switzerland

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