Large-Scale, Quantitative Protein Assays on a High-Throughput DNA Sequencing Chip.

Layton, Curtis J; McMahon, Peter L; Greenleaf, William J

Layton, Curtis J; McMahon, Peter L; Greenleaf, William J.

Affiliation

Layton CJ; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
McMahon PL; Department of Applied Physics, Stanford University, Stanford, CA 94305, USA.
Greenleaf WJ; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Applied Physics, Stanford University, Stanford, CA 94305, USA; Chan-Zuckerberg Initiative, Palo Alto, CA 94301, USA. Electronic address: wjg@stanford.edu.

Mol Cell ; 73(5): 1075-1082.e4, 2019 03 07.

Article in En | MEDLINE | ID: mdl-30849388

Subject(s)

Antibodies/metabolism; DNA Mutational Analysis/methods; High-Throughput Nucleotide Sequencing/methods; O(6)-Methylguanine-DNA Methyltransferase/metabolism; Oligonucleotide Array Sequence Analysis/methods; Oligopeptides/metabolism; Protein Array Analysis/methods; Antibody Affinity; Antibody Specificity; Automation, Laboratory; Binding Sites, Antibody; Catalysis; DNA Mutational Analysis/instrumentation; High-Throughput Nucleotide Sequencing/instrumentation; Kinetics; Mutation; O(6)-Methylguanine-DNA Methyltransferase/genetics; Oligonucleotide Array Sequence Analysis/instrumentation; Oligopeptides/genetics; Protein Array Analysis/instrumentation; Protein Binding; Protein Engineering; Workflow

Key words

SNAP tag; antibody characterization; high-throughput; in vitro translation; protein array; protein engineering; protein evolution; ribosome display; sequencer hacking; superFLAG

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligopeptides / DNA Mutational Analysis / O(6)-Methylguanine-DNA Methyltransferase / Oligonucleotide Array Sequence Analysis / Protein Array Analysis / High-Throughput Nucleotide Sequencing / Antibodies Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article Affiliation country: United States

Fulltext

XML

PubMed Links

Search on Google