Development of a Modified Surgical Technique for Simulating Ischemic Cerebral Cortex Injury in Rats.
In Vivo
; 33(4): 1175-1181, 2019.
Article
in En
| MEDLINE
| ID: mdl-31280207
ABSTRACT
BACKGROUND/AIM:
Middle cerebral artery occlusion (MCAO) in rodents is an essential animal model for research focusing on ischemic stroke. To date, several kinds of surgical methods for MCAO have been developed and the craniotomy method has the advantage of direct visualization of the middle cerebral artery (MCA). MCAO at a more proximal site produces better surgical results, but it is a more invasive technique. The aim of this study was to evolve the surgical technique for simulating ischemic cerebral cortex injury in rats. MATERIALS ANDMETHODS:
To approach proximal MCA with a less invasive procedure, a modified surgical technique for MCAO in rats was developed. Besides, rats receiving the modified and conventional method were compared with regard to infarct volume and by behavioral tests.RESULTS:
Following craniotomy, we proposed that the inferior edge of the craniotomy should be enlarged with fine forceps. This modified surgical method induces larger infarct volume, significant behavioral impairment and can induce ischemic stroke. Additionally, it does not significantly increase the operation time, and has produced no obvious complications.CONCLUSION:
This modified surgical technique may serve as a practical method for performing MCAO.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Ischemia
/
Neurosurgical Procedures
/
Disease Models, Animal
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
In Vivo
Journal subject:
NEOPLASIAS
Year:
2019
Type:
Article