Spinal miR-34a regulates inflammatory pain by targeting SIRT1 in complete Freund's adjuvant mice.
Biochem Biophys Res Commun
; 516(4): 1196-1203, 2019 09 03.
Article
in En
| MEDLINE
| ID: mdl-31296380
ABSTRACT
Sirtuin1 (SIRT1), which is regulated by microRNA-34a (miR-34a), can modulate pathophysiology processes, including nonalcoholic fatty liver disease and intestinal ischemia/reperfusion injury. We previously reported that SIRT1, an NAD+-dependent deacetylase, plays a vital role in the development of neuropathic pain. However, the role of miR-34a/SIRT1 in complete Freund's adjuvant (CFA)-induced inflammatory pain remains unclear. In the present study, we examined miR-34a and SIRT1 in CFA mice. MiR-34a levels increased, while SIRT1 decreased in the spinal cord. Inhibiting miR-34a by intrathecal injection of miR-34a antagomir attenuated CFA-induced pain behavior. Moreover, miR-34a antagomir inhibited the CFA-induced SIRT1 decrease in the spinal cord. Furthermore, the analgesic effect of miR-34a antagomir was abrogated by the SIRT1 inhibitor EX-527. Our data provide support that the underlying mechanisms of miR-34a in promoting inflammatory pain may involve negative regulation of SIRT1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pain
/
Spinal Cord
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MicroRNAs
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Sirtuin 1
/
Inflammation
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2019
Type:
Article
Affiliation country:
China