Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.

Yao, Run-Wen; Xu, Guang; Wang, Ying; Shan, Lin; Luan, Peng-Fei; Wang, Yang; Wu, Man; Yang, Liang-Zhong; Xing, Yu-Hang; Yang, Li; Chen, Ling-Ling

Yao, Run-Wen; Xu, Guang; Wang, Ying; Shan, Lin; Luan, Peng-Fei; Wang, Yang; Wu, Man; Yang, Liang-Zhong; Xing, Yu-Hang; Yang, Li; Chen, Ling-Ling.

Affiliation

Yao RW; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Xu G; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Wang Y; CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Shan L; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Luan PF; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Wang Y; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Wu M; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Yang LZ; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Xing YH; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000
Yang L; CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China;
Chen LL; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 2000

Mol Cell ; 76(5): 767-783.e11, 2019 12 05.

Article in En | MEDLINE | ID: mdl-31540874

Subject(s)

Cell Nucleolus/metabolism; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional; RNA, Ribosomal/metabolism; Active Transport, Cell Nucleus; Antigens, Nuclear/genetics; Antigens, Nuclear/metabolism; Cell Nucleolus/genetics; Cell Nucleolus/ultrastructure; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Female; HEK293 Cells; HeLa Cells; Humans; Nucleic Acid Conformation; Protein Binding; Protein Interaction Domains and Motifs; RNA Precursors/genetics; RNA Precursors/ultrastructure; RNA, Ribosomal/genetics; RNA, Ribosomal/ultrastructure

Key words

Fibrillarin; RNA binding domain; RNA sorting; condensates; glycine- and arginine-rich; intrinsically disordered region; nucleolus; phase separation; ribosomal RNA; super-resolution microscopy

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Ribosomal / RNA Precursors / Cell Nucleolus / RNA Processing, Post-Transcriptional Limits: Female / Humans Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article

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