Identification of lncRNA NEAT1/miR-21/RRM2 axis as a novel biomarker in breast cancer.
J Cell Physiol
; 235(4): 3372-3381, 2020 04.
Article
in En
| MEDLINE
| ID: mdl-31621912
ABSTRACT
As a major disease that threatens the health of women worldwide, breast cancer (BC) lacks effective molecular markers in the clinic at the same time. We aim at finding a new biomarker of BC. In our study, through the Gene Expression Omnibus database chip, a total of 1393 pairs of microRNA-messenger RNA (miRNA-mRNA) networks and 35754 pairs of long noncoding RNA-miRNA networks were obtained. We found out that NEAT1/miR-21/RRM2 axis may play a role in BC diagnosis and prognosis. The real-time quantitative reverse transcription-polymerase chain reaction test was used to analyze the mRNA level of NEAT1, miR-21, and RRM2. Western blot was used to detect the protein level of RRM2. Through the 5-ethynyl-2'-deoxyuridine assay, the proliferation of MDA-MB-231 cells was detected. Through wound healing and transwell assay, the migration of MDA-MB-231 cells was detected. Altogether, our data indicated that NEAT1, miR-21, and RRM2 were upregulated in several BC cell lines. Overexpressed of miR-21 in MDA-MB-231 cells promote proliferation and migration. Besides, our results demonstrated that overexpressed of miR-21 upregulated the level of RRM2. Accordingly, miR-21/RRM2 might be a new diagnosis and treatment target of BC.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribonucleoside Diphosphate Reductase
/
Breast Neoplasms
/
MicroRNAs
/
RNA, Long Noncoding
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
J Cell Physiol
Year:
2020
Type:
Article
Affiliation country:
China