Production, optimization, purification, characterization, and anti-cancer application of extracellular L-glutaminase produced from the marine bacterial isolate.

Orabi, Hanaa; El-Fakharany, Esmail; Abdelkhalek, Eman; Sidkey, Nagwa

Orabi, Hanaa; El-Fakharany, Esmail; Abdelkhalek, Eman; Sidkey, Nagwa.

Affiliation

Orabi H; Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications (SRTA-City), Alexandria, Egypt.
El-Fakharany E; Faculty of Science, Botany and Microbiology Department, Al-Azhar University, Cairo, Egypt.
Abdelkhalek E; Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications (SRTA-City), Alexandria, Egypt.
Sidkey N; Faculty of Science, Botany and Microbiology Department, Al-Azhar University, Cairo, Egypt.

Prep Biochem Biotechnol ; 50(4): 408-418, 2020.

Article in En | MEDLINE | ID: mdl-31846380

Subject(s)

Antineoplastic Agents/pharmacology; Glutaminase/pharmacology; Animals; Antineoplastic Agents/chemistry; Antineoplastic Agents/isolation & purification; Apoptosis/drug effects; Bacillus subtilis/enzymology; Bacillus subtilis/isolation & purification; Cell Line, Tumor; Chlorocebus aethiops; Drug Screening Assays, Antitumor; Enzyme Assays; Enzyme Stability; Glutaminase/chemistry; Glutaminase/isolation & purification; Humans; Mice; Temperature; Vero Cells

Key words

Apoptosis; L-glutaminase; characterization; optimization; purification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glutaminase / Antineoplastic Agents Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Prep Biochem Biotechnol Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2020 Type: Article Affiliation country: Egypt

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