Personalized Mapping of Drug Metabolism by the Human Gut Microbiome.
Cell
; 181(7): 1661-1679.e22, 2020 06 25.
Article
in En
| MEDLINE
| ID: mdl-32526207
ABSTRACT
The human gut microbiome harbors hundreds of bacterial species with diverse biochemical capabilities. Dozens of drugs have been shown to be metabolized by single isolates from the gut microbiome, but the extent of this phenomenon is rarely explored in the context of microbial communities. Here, we develop a quantitative experimental framework for mapping the ability of the human gut microbiome to metabolize small molecule drugs Microbiome-Derived Metabolism (MDM)-Screen. Included are a batch culturing system for sustained growth of subject-specific gut microbial communities, an ex vivo drug metabolism screen, and targeted and untargeted functional metagenomic screens to identify microbiome-encoded genes responsible for specific metabolic events. Our framework identifies novel drug-microbiome interactions that vary between individuals and demonstrates how the gut microbiome might be used in drug development and personalized medicine.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drug Evaluation, Preclinical
/
Microbiota
/
Gastrointestinal Microbiome
Limits:
Adult
/
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Cell
Year:
2020
Type:
Article
Affiliation country:
United States