EDTA-FISH: A Simple and Effective Approach to Reduce Non-specific Adsorption of Probes in Fluorescence in situ Hybridization (FISH) for Environmental Samples.

Morono, Yuki; Kubota, Kengo; Tsukagoshi, Daisuke; Terada, Takeshi

Morono, Yuki; Kubota, Kengo; Tsukagoshi, Daisuke; Terada, Takeshi.

Affiliation

Morono Y; Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Earth-Marine Science and Technology (JAMSTEC).
Kubota K; Department of Civil and Environmental Engineering, Tohoku University.
Tsukagoshi D; Department of Civil and Environmental Engineering, Tohoku University.
Terada T; Marine Works Japan LTD.

Microbes Environ ; 35(3)2020.

Article in En | MEDLINE | ID: mdl-32595184

Subject(s)

Edetic Acid/chemistry; In Situ Hybridization, Fluorescence/methods; Oligonucleotide Probes/chemistry; Adsorption; Fluorescence; Geologic Sediments/microbiology; Indicators and Reagents/chemistry; Seawater/microbiology

Key words

EDTA; Fluorescence in situ hybridization (FISH); marine sediments

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotide Probes / Edetic Acid / In Situ Hybridization, Fluorescence Language: En Journal: Microbes Environ Year: 2020 Type: Article

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