C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori.
J Invertebr Pathol
; 179: 107473, 2021 02.
Article
in En
| MEDLINE
| ID: mdl-32946913
ABSTRACT
The Janus kinase/signal transducer and activator of transcription cascade transduction (JAK/STAT) signaling pathway is highly conserved in mammals, but the pattern recognition receptors (PRRs) and their functions are unclear. We found that the expression pattern of Bombyx mori C-type lectin 5 (BmCTL 5) had a synergy relevance with the JAK/STAT signaling pathway against Beauveria bassiana. An RNAi assay, subcellular localization analysis, yeast two-hybrid technique, protein recruitment experiment and pathogen infection tests were used to explore the roles of BmCTL 5 in the JAK/STAT signaling pathway. Knock-down of the BmCTL 5 suppressed the JAK/STAT signaling pathway and the PO cascade of nodule melanization. BmCTL 5 is located in the cytomembrane and interacted with BmHOP both in yeast and B. mori ovary cells N (BmN cells). BmCTL 5 and the JAK/STAT signaling pathway was activated by B. bassiana but only slightly activated by B. mori cytoplasmic polyhedrosis virus (BmCPV), Nosema bombycis and bacteria LPS. These findings suggest that BmCTL 5 might be an important PRR for the JAK/STAT signaling pathway and may mediate the nodule melanization for fungi infection. These data provide insights into the immune mechanism of the JAK/STAT signaling pathway in insects and aid understanding of the mechanism of the JAK/STAT signaling pathway and adaptive immune systems in mammals.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bombyx
/
Signal Transduction
/
Insect Proteins
/
Lectins, C-Type
/
Receptors, Pattern Recognition
/
Beauveria
Limits:
Animals
Language:
En
Journal:
J Invertebr Pathol
Year:
2021
Type:
Article
Affiliation country:
China