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Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression.
Ito, Yoichiro; Terai, Goro; Ishigami, Misa; Hashiba, Noriko; Nakamura, Yasuyuki; Bamba, Takahiro; Kumokita, Ryota; Hasunuma, Tomohisa; Asai, Kiyoshi; Ishii, Jun; Kondo, Akihiko.
Affiliation
  • Ito Y; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan.
  • Terai G; Engineering Biology Research Center, Kobe University, Kobe 657-8501, Japan.
  • Ishigami M; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8561, Japan.
  • Hashiba N; Technology Research Association of Highly Efficient Gene Design, Kobe 650-0047, Japan.
  • Nakamura Y; Technology Research Association of Highly Efficient Gene Design, Kobe 650-0047, Japan.
  • Bamba T; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan.
  • Kumokita R; Engineering Biology Research Center, Kobe University, Kobe 657-8501, Japan.
  • Hasunuma T; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan.
  • Asai K; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan.
  • Ishii J; Graduate School of Science, Technology and Innovation, Kobe University, Kobe 657-8501, Japan.
  • Kondo A; Engineering Biology Research Center, Kobe University, Kobe 657-8501, Japan.
Nucleic Acids Res ; 48(22): 13000-13012, 2020 12 16.
Article in En | MEDLINE | ID: mdl-33257988
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Terminator Regions, Genetic / RNA Stability / Saccharomycetales Language: En Journal: Nucleic Acids Res Year: 2020 Type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Terminator Regions, Genetic / RNA Stability / Saccharomycetales Language: En Journal: Nucleic Acids Res Year: 2020 Type: Article