High-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing.
Nat Methods
; 18(2): 165-169, 2021 02.
Article
in En
| MEDLINE
| ID: mdl-33432244
ABSTRACT
High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies (ONT) or Pacific Biosciences circular consensus sequencing, yielding high-accuracy single-molecule consensus sequences of large genomic regions. We applied our approach to sequence ribosomal RNA operon amplicons (~4,500 bp) and genomic sequences (>10,000 bp) of reference microbial communities in which we observed a chimera rate <0.02%. To reach a mean UMI consensus error rate <0.01%, a UMI read coverage of 15× (ONT R10.3), 25× (ONT R9.4.1) and 3× (Pacific Biosciences circular consensus sequencing) is needed, which provides a mean error rate of 0.0042%, 0.0041% and 0.0007%, respectively.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
High-Throughput Nucleotide Sequencing
/
Nanopores
/
Microbiota
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2021
Type:
Article
Affiliation country:
Denmark