Impact of pesticide/fertilizer mixtures on the rhizosphere microbial community of field-grown sugarcane.

ABSTRACT

The rhizosphere microbial community is important for plant health and is shaped by numerous environmental factors. This study aimed to unravel the effects of a pesticide/fertilizer mixture on the soil rhizosphere microbiome of field-grown sugarcane. A field trial on sugarcane was conducted in Zhanjian City, Guangdong Province, China, and soil samples from the rhizosphere were collected after clothianidin pesticide and/or organic fertilizer treatments. The effects of pesticide and/or organic fertilizer treatments on the composition, diversity, and predictive function of the rhizosphere microbial communities were examined using 16S rRNA gene and ITS1 amplicon sequencing. Compared with the controls (no pesticide or fertilizer used), the microbial community that resulted from treatment with the pesticide/fertilizer mixture (SPF) had a higher relative bacterial diversity and fungal richness, and contributed more beneficial functions to sugarcane, including xenobiotics biodegradation and metabolism of amino acids. The bacterial and fungal compositions at various taxonomic levels were not significantly different in SPF and SP (pesticide only) treatments compared to treatments without the pesticide, suggesting that the clothianidin addition did not cause a detrimental impact on the soil microbiome. Moreover, five bacterial genera, including Dyella, Sphingomonas, Catenulispora, Mucilaginibacter, and Tumebacillus, were significantly more abundant in the SPF and SP treatments, which could be associated with the pesticide addition. With the addition of organic fertilizers in SPF, the abundances of some soil-beneficial bacteria Bacillus, Paenibacillus, and Brevibacillus were highly increased. Our study provides insights into the interactions between the rhizosphere soil microbiome and pesticide-fertilizer integration, which may help improve the application of pesticide-fertilizer to sugarcane fields. SUPPLEMENTARY INFORMATION The online version contains supplementary material available at 10.1007/s13205-021-02770-3.