Identification of the human beta-actin enhancer and its binding factor.
Mol Cell Biol
; 8(1): 267-72, 1988 Jan.
Article
in En
| MEDLINE
| ID: mdl-3422098
ABSTRACT
An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759 and +783 downstream from the cap site) with high specificity. This finding correlated with those of DNase I protection and exonuclease III digestion assays. This binding region of the beta-actin enhancer contained a hyphenated dyad symmetry and an enhancer core-like sequence. In vitro competition experiments indicated that the factor did not bind to the simian virus 40 enhancer core region.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Actins
/
Enhancer Elements, Genetic
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Mol Cell Biol
Year:
1988
Type:
Article
Affiliation country:
Japan