CRISPRi/a Screening with Human iPSCs.

Nishiga, Masataka; Qi, Lei S; Wu, Joseph C

Nishiga, Masataka; Qi, Lei S; Wu, Joseph C.

Affiliation

Nishiga M; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
Qi LS; Department of Bioengineering, Stanford University School of Medicine, Stanford, CA, USA.
Wu JC; Department of Chemical & Systems Biology, Stanford University School of Medicine, Stanford, CA, USA.

Methods Mol Biol ; 2320: 261-281, 2021.

Article in En | MEDLINE | ID: mdl-34302664

Subject(s)

CRISPR-Cas Systems; Gene Editing/methods; Induced Pluripotent Stem Cells/cytology; Base Sequence; Causality; Cells, Cultured; Chromatography, Liquid/methods; DNA/isolation & purification; Doxycycline/pharmacology; Flow Cytometry; Genetic Association Studies; Genetic Vectors/genetics; HEK293 Cells; High-Throughput Nucleotide Sequencing/methods; Humans; Lentivirus/genetics; Myocytes, Cardiac/cytology; Myocytes, Cardiac/metabolism; RNA, Guide, Kinetoplastida/genetics; Transfection

Key words

CRISPR/Cas9; Cardiomyocytes; Genome editing; Induced pluripotent stem cells

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Induced Pluripotent Stem Cells / CRISPR-Cas Systems / Gene Editing Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Type: Article Affiliation country: United States

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