SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs.
Genome Biol
; 22(1): 277, 2021 09 23.
Article
in En
| MEDLINE
| ID: mdl-34556162
ABSTRACT
Here we describe a new integrative approach for accurate annotation and quantification of circRNAs named Short Read circRNA Pipeline (SRCP). Our strategy involves two steps:
annotation of validated circRNAs followed by a quantification step. We show that SRCP is more sensitive than other individual pipelines and allows for more comprehensive quantification of a larger number of differentially expressed circRNAs. To facilitate the use of SRCP, we generate a comprehensive collection of validated circRNAs in five different organisms, including humans. We then utilize our approach and identify a subset of circRNAs bound to the miRNA-effector protein AGO2 in human brain samples.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Software
/
Molecular Sequence Annotation
/
RNA, Circular
Limits:
Animals
/
Humans
Language:
En
Journal:
Genome Biol
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2021
Type:
Article
Affiliation country:
Israel