Partial purification of a nuclear protein that binds to the CCAAT box of the mouse alpha 1-globin gene.
Mol Cell Biol
; 6(3): 821-32, 1986 Mar.
Article
in En
| MEDLINE
| ID: mdl-3464831
ABSTRACT
We enriched a fraction from nuclear extracts of murine erythroleukemia cells which contains a protein able to form stable complexes with the promoter region of the alpha 1-globin gene. Binding activity, which is present in mouse brain and a variety of cultured mouse and human cell lines, is not erythroid cell specific. Binding studies with alpha-globin gene promoter deletion mutants as well as DNase I footprinting and dimethyl sulfate protection studies showed that the factor bound specifically to the CCAAT box of the alpha 1 promoter. Enriched factor preparations exhibited weak binding to the promoter region of the beta maj-globin gene. This suggests that this protein could bind differentially to these two promoters in vivo. The enriched factor may be a ubiquitous nuclear protein involved in the differential regulation of the alpha 1- and beta maj-globin genes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Globins
/
DNA-Binding Proteins
/
Genes
/
Nucleoproteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Cell Biol
Year:
1986
Type:
Article