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miR-874-3p mitigates cisplatin resistance through modulating NF-κB/inhibitor of apoptosis protein signaling pathway in epithelial ovarian cancer cells.
Wang, Ying; Yan, Chenming; Qi, Junxia; Liu, Chunyan; Yu, Juan; Wang, Huabin.
Affiliation
  • Wang Y; Department of Obstetrics and Gynecology, Sichuan Tianfu New Area People's Hospital, Chengdu, China.
  • Yan C; Department of Obstetrics and Gynecology, Cixian People's Hospital, Cixian, China.
  • Qi J; Department of Obstetrics and Gynecology, Handan PuHui Obstetrics and Gynecology Hospital, Handan, China.
  • Liu C; Department of Obstetrics and Gynecology, Sichuan Tianfu New Area People's Hospital, Chengdu, China.
  • Yu J; Department of Obstetrics and Gynecology, Sichuan Tianfu New Area People's Hospital, Chengdu, China.
  • Wang H; Department of Gynecology, Jintang County Maternity and Child Health Hospital, No. 6, Jinquan Road, Jintang County, Chengdu, China. huab.wang@yahoo.com.
Mol Cell Biochem ; 477(1): 307-317, 2022 Jan.
Article in En | MEDLINE | ID: mdl-34716858
ABSTRACT
The resistance to cisplatin, the most common platinum chemotherapy drug, may confine the efficacy of treatment in epithelial ovarian cancer patients. Aberrant expression of inhibitor of apoptosis proteins set the stage for resistance to cisplatin in EOC; besides, chemosensitivity in EOC can be chalked up to dysregulation of specific miRNAs. Herein, we investigated whether there is a potential correlation between miR-874-3p and the X-chromosome-linked inhibitor of apoptosis, a member of the IAP protein family in cisplatin-resistant EOC cells. The lower expression of miR-874-3p was found in SKOV3-DDP cells; it was also in association with cisplatin-resistance in EOC cells. XIAP was found to contribute to developing platinum resistance and is an authentic target for miR-874-3p in SKOV3-DDP cells. Consistently, restoration of miR-874-3p expression reversed cisplatin resistance in such cells by modulating XIAP and NF-κB/Survivin signaling pathway. Besides, siRNA knock down of XIAP in SKOV3-DDP cells had an anti-migratory effect like those with miR-874 overexpression. Importantly, the enforced expression of XIAP rescued SKOV3-DDP cells from the cytotoxic effects of miR-874-3p. Finally, miR-874-3p sensitized EOC cells to cisplatin-induced apoptosis, at least in part, through targeting XIAP. The cytotoxic effects of miR-874-3p can be attributed to the targeting XIAP in cisplatin-resistant EOC cells. We believe that the combination of cisplatin with miR-874-3p may make a potential strategy to reverse cisplatin resistance.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ovarian Neoplasms / RNA, Neoplasm / Signal Transduction / NF-kappa B / Cisplatin / Apoptosis / Drug Resistance, Neoplasm / MicroRNAs / Carcinoma, Ovarian Epithelial / Neoplasm Proteins Limits: Female / Humans Language: En Journal: Mol Cell Biochem Year: 2022 Type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ovarian Neoplasms / RNA, Neoplasm / Signal Transduction / NF-kappa B / Cisplatin / Apoptosis / Drug Resistance, Neoplasm / MicroRNAs / Carcinoma, Ovarian Epithelial / Neoplasm Proteins Limits: Female / Humans Language: En Journal: Mol Cell Biochem Year: 2022 Type: Article Affiliation country: China