Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag.

Fujiwara, Yasuhiro; Tanno, Yuji; Sugishita, Hiroki; Kishi, Yusuke; Makino, Yoshinori; Okada, Yuki

Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag.

Fujiwara, Yasuhiro; Tanno, Yuji; Sugishita, Hiroki; Kishi, Yusuke; Makino, Yoshinori; Okada, Yuki.

Affiliation

Fujiwara Y; Institute of Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Tanno Y; Bioscience Department, VERITAS Corporation, Tokyo, Japan.
Sugishita H; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Kishi Y; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Makino Y; Institute of Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Okada Y; Institute of Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.

PLoS One ; 16(11): e0259846, 2021.

Article in En | MEDLINE | ID: mdl-34784358

Subject(s)

Concanavalin A/administration & dosage; Histones/metabolism; Staphylococcal Protein A/genetics; Transposases/genetics; Animals; Cell Line; Concanavalin A/chemistry; Concanavalin A/pharmacology; Epigenomics; HEK293 Cells; Histone Code; Humans; Immunomagnetic Separation; Magnetic Fields; Methylation; Mice; Particle Size; Recombinant Fusion Proteins/metabolism; Staphylococcal Protein A/metabolism; Transposases/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcal Protein A / Histones / Concanavalin A / Transposases Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2021 Type: Article Affiliation country: Japan

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