Normal rhodanese activity in leukocytes from Leber patients: enzyme characterization and activity levels.
Neurology
; 37(12): 1878-80, 1987 Dec.
Article
in En
| MEDLINE
| ID: mdl-3479705
ABSTRACT
The thiosulfatecyanide sulfurtransferase (rhodanese) enzyme (EC 2.8.1.1.) was studied in human leukocytes from control subjects and from nine patients with Leber's hereditary optic atrophy. Enzyme activity was proportional to protein concentration in the tested range (0.09 to 0.39 mg) as well as to incubation time. The optimal pH for reaction was 8.7 and the apparent Km for thiosulfate was 7 X 10(-3) M. No significant difference of enzyme activity was present in Leber's disease.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfurtransferases
/
Thiosulfate Sulfurtransferase
/
Optic Atrophy
/
Genetic Diseases, Inborn
/
Leukocytes
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Neurology
Year:
1987
Type:
Article
Affiliation country:
Italy