Synthesis and application of POLYseq for profiling human liver organoids.

Dunn, Andrew W; Cai, Yuqi; Iwasawa, Kentaro; Kimura, Masaki; Takebe, Takanori

Dunn, Andrew W; Cai, Yuqi; Iwasawa, Kentaro; Kimura, Masaki; Takebe, Takanori.

Affiliation

Dunn AW; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology and Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Cai Y; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology and Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Iwasawa K; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology and Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Kimura M; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology and Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Takebe T; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology and Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

STAR Protoc ; 2(4): 100976, 2021 12 17.

Article in En | MEDLINE | ID: mdl-34934958

Subject(s)

Liver/cytology; Optical Imaging/methods; Organoids/cytology; Sequence Analysis, DNA/methods; Single-Cell Analysis/methods; Humans

Key words

Biotechnology and bioengineering; Cell-based Assays; Chemistry; Organoids; RNAseq; Sequencing; Single Cell; Stem Cells

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoids / Sequence Analysis, DNA / Single-Cell Analysis / Optical Imaging / Liver Limits: Humans Language: En Journal: STAR Protoc Year: 2021 Type: Article Affiliation country: United States

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