Deficiency of ribosomal proteins reshapes the transcriptional and translational landscape in human cells.
Nucleic Acids Res
; 50(12): 6601-6617, 2022 07 08.
Article
in En
| MEDLINE
| ID: mdl-35137207
Ribosomes are the main effector of the translational machinery to synthesize proteins. In this study, the authors characterized genome-wide transcriptional and translational changes after knocking-down 75 individual human ribosomal proteins (RPs). They revealed that deficiency of individual RPs perturbed expression of specific subsets of genes, enriched in eight major functional classes, such as cell cycle and development. RPs were subjected to co-translational regulation under ribosomal stress where deficiency of the 60S RPs and the 40S RPs had opposite effects on the two subunits. They also showed that RPS8 deficiency stimulated cellular apoptosis while RPL13 and RPL18 deficiency promoted cellular senescence. They further showed functional and regulatory roles of RPL11 and RPL15 in retina development and angiogenesis, respectively.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribosomal Proteins
/
Ribosome Subunits, Large, Eukaryotic
/
Ribosome Subunits, Small, Eukaryotic
Limits:
Humans
Language:
En
Journal:
Nucleic Acids Res
Year:
2022
Type:
Article
Affiliation country:
China