Establishment of a High Content Image Platform to Measure NF-&#954;B Nuclear Translocation in LPS-induced RAW264.7 Macrophages for Screening Anti-inflammatory Drug Candidates.

Zhang, Yan-Yu; Yao, Yun-Da; Cheng, Qi-Qing; Huang, Yu-Feng; Zhou, Hua

Establishment of a High Content Image Platform to Measure NF-κB Nuclear Translocation in LPS-induced RAW264.7 Macrophages for Screening Anti-inflammatory Drug Candidates.

Zhang, Yan-Yu; Yao, Yun-Da; Cheng, Qi-Qing; Huang, Yu-Feng; Zhou, Hua.

Affiliation

Zhang YY; Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, P.R. China.
Yao YD; Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Guangzhou University of Chinese Medicine, Guangzhou 510006, P.R. China.
Cheng QQ; Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, P.R. China.
Huang YF; Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Guangzhou University of Chinese Medicine, Guangzhou 510006, P.R. China.
Zhou H; Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, P.R. China.

Curr Drug Metab ; 23(5): 394-414, 2022 08 03.

Article in En | MEDLINE | ID: mdl-35410593

Subject(s)

Lipopolysaccharides; NF-kappa B; Anti-Inflammatory Agents/pharmacology; Humans; Inflammation/metabolism; Interleukin-6/metabolism; Interleukin-6/pharmacology; Lipopolysaccharides/metabolism; Lipopolysaccharides/pharmacology; Macrophages/metabolism; NF-kappa B/genetics; Nitric Oxide/metabolism; Nitric Oxide/pharmacology; RNA, Messenger/metabolism; Reproducibility of Results; Tumor Necrosis Factor-alpha/genetics; Tumor Necrosis Factor-alpha/metabolism; Tumor Necrosis Factor-alpha/pharmacology

Key words

BAY 11-7085; High content image; RAW264.7 macrophages; anti-inflammation; benzoylhypaconine; lipopolysaccharide; nuclear factor-κB nuclear translocation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lipopolysaccharides / NF-kappa B Type of study: Diagnostic_studies / Screening_studies Limits: Humans Language: En Journal: Curr Drug Metab Journal subject: METABOLISMO / QUIMICA Year: 2022 Type: Article

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