Rapid detection of sweepoviruses through lateral flow dipstick-based recombinase polymerase amplification.
Acta Virol
; 66(2): 186-191, 2022.
Article
in En
| MEDLINE
| ID: mdl-35766476
ABSTRACT
Sweepoviruses represent a phylogenetic group of begomoviruses that cause significant sweet potato (Ipomoea batatas) production losses in various countries across the world. For rapid identification of sweepoviruses, we developed a technique based on isothermal recombinase polymerase amplification in conjunction with lateral flow dipsticks (RPA-LFD). The optimum reaction conditions for the RPA were 20 min incubation at 37°C. The RPA-LFD specifically detected distinct sweepovirus species, with no other viruses infecting sweet potato causing a cross-reaction. The detection limit of the RPA-LFD was 1.0×104 copies of the target DNA molecule per reaction, and it exhibited a 10-fold greater sensitivity than the conventional PCR. Furthermore, when coupled with an alkaline polyethylene glycol-based crude genomic DNA extraction, the entire procedure was completed in 30 min without the use of any special instruments other than a water bath. Therefore, the RPA-LFD technique is a potential sweepovirus diagnostic tool that can be used in the field with fewer available resources. Keywords detection; sweepoviruses; recombinase polymerase amplification; lateral flow dipstick.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acid Amplification Techniques
/
Recombinases
Type of study:
Diagnostic_studies
Language:
En
Journal:
Acta Virol
Year:
2022
Type:
Article