A cell penetrating peptide-modified magnetic/fluorescent probe for in vivo tracking of mesenchymal stem cells.
J Biomed Mater Res A
; 110(12): 1881-1891, 2022 12.
Article
in En
| MEDLINE
| ID: mdl-35852385
ABSTRACT
Difficulty in precise tracking of the in vivo distribution and migration of transplanted stem cells in a noninvasive way is a great hurdle in regenerative medicine research. Paramagnetic element-doped upconversion nanoparticles (UCNPs) emerge as a kind of promising dual-modal imaging agents owing to the possibility in combining strong contrast of MR imaging and high efficiency of fluorescence imaging. Herein a kind of Mn-doped NaYF4-based UCNPs (NaYF4 20%Yb, 2%Er, 30%Mn) was developed and then hydrophilized by various coatings, including cell penetrating peptide, DNA, and SiO2 . The UCNPs were denoted as Pep/UCNPs, DNA/UCNPs, and SiO2 /UCNPs, respectively. Their potential in cell labeling and in vivo tracking applications was comprehensively explored. The results show that the Mn-doped UCNPs possess high biocompatibility, high cell labeling efficiency, high MR resolution, and importantly a single emission at 660 nm, within optical window for in vivo imaging of biological targets. Particularly, mesenchymal stem cells (MSCs) pre-labeled by cell penetrating peptide-modified UCNPs (Pep/UCNPs) can be precisely monitored in terms of their distribution in mice over a long period of time by simultaneous MR and fluorescent imaging, which provided a noninvasive and double-checking tool for investigating the destination of stem cells in tissue regeneration.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanoparticles
/
Cell-Penetrating Peptides
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
En
Journal:
J Biomed Mater Res A
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2022
Type:
Article
Affiliation country:
China