DNA banking: the effects of storage of blood and isolated DNA on the integrity of DNA.
Am J Med Genet
; 27(2): 379-90, 1987 Jun.
Article
in En
| MEDLINE
| ID: mdl-3605221
ABSTRACT
Long-term storage of DNA is required for a number of genetic studies; prior to extraction, blood samples may be subject to elevated temperatures for variable intervals. We have studied the effect of temperatures ranging from -70 degrees C to +65 degrees C on human blood and on DNA extracted from it. DNA in solution stored at ambient temperatures up to 37 degrees C for 6 months was digestible by three different restriction endonucleases, whereas storage at 45 degrees C is deleterious after 6-7 weeks. DNA can be extracted from blood samples stored at -70 degrees C for at least 2 months or at 23 degrees C for a week or more, but blood stored at these temperatures may yield less high-molecular-weight DNA. Cell pellets from which plasma has been removed also can serve as a source of DNA. Isolated DNA stored dry for years (up to 30) is difficult to dissolve and may appear degraded, but a sample stored dry for 13 years and then in solution at -20 degrees C for 7 years appeared to be intact.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Banks
/
Blood Preservation
/
DNA Damage
/
DNA
Type of study:
Observational_studies
Limits:
Humans
Language:
En
Journal:
Am J Med Genet
Year:
1987
Type:
Article