Biosynthesis of the Isocoumarin Derivatives Fusamarins is Mediated by the PKS8 Gene Cluster in Fusarium.
Chembiochem
; 24(6): e202200342, 2023 03 14.
Article
in En
| MEDLINE
| ID: mdl-36137261
ABSTRACT
Fusarium mangiferae causes the mango malformation disease (MMD) on young mango trees and seedlings resulting in economically significant crop losses. In addition, F.â
mangiferae produces a vast array of secondary metabolites (SMs), including mycotoxins that may contaminate the harvest. Their production is tightly regulated at the transcriptional level. Here, we show that lack of the H3â
K9-specific histone methyltransferase, FmKmt1, influences the expression of the F.â
mangiferae polyketide synthase (PKS) 8 (FmPKS8), a so far cryptic PKS. By a combination of reverse genetics, untargeted metabolomics, bioinformatics and chemical analyses including structural elucidation, we determined the FmPKS8 biosynthetic gene cluster (BGC) and linked its activity to the production of fusamarins (FMN), which can be structurally classified as dihydroisocoumarins. Functional characterization of the four FMN cluster genes shed light on the biosynthetic pathway. Cytotoxicity assays revealed moderate toxicities with IC50 values between 1 and 50â
µM depending on the compound.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mangifera
/
Fusarium
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2023
Type:
Article
Affiliation country:
Austria