Your browser doesn't support javascript.
loading
A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin ß1 Interactomes.
Song, Didi-Andreas; Alber, Stefanie; Doron-Mandel, Ella; Schmid, Vera; Albus, Christin A; Leitner, Orith; Hamawi, Hedva; Oses-Prieto, Juan A; Dezorella, Nili; Burlingame, Alma L; Fainzilber, Mike; Rishal, Ida.
Affiliation
  • Song DA; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Alber S; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Doron-Mandel E; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Schmid V; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Albus CA; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Leitner O; Life Science Core Facilities, Faculty of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
  • Hamawi H; Life Science Core Facilities, Faculty of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
  • Oses-Prieto JA; Department of Pharmaceutical Chemistry, University of California, San Francisco, California, USA.
  • Dezorella N; Electron Microscopy Unit, Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.
  • Burlingame AL; Department of Pharmaceutical Chemistry, University of California, San Francisco, California, USA.
  • Fainzilber M; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel.
  • Rishal I; Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot, Israel. Electronic address: ida.rishal@weizmann.ac.il.
Mol Cell Proteomics ; 21(11): 100418, 2022 11.
Article in En | MEDLINE | ID: mdl-36180036
ABSTRACT
Importin ß1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in the detection of importin complex cargos and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable biotinylation by antibody recognition analysis of importin ß1 interactomes. The monoclonal antibody recognizes an epitope comprising residues 301-320 of human KPBN1 and strikingly is highly specific for cytoplasmic KPNB1 in diverse applications, with little reaction with KPNB1 in the nucleus. Biotinylation by antibody recognition with this novel antibody revealed numerous new interactors of importin ß1, expanding the KPNB1 interactome to cytoplasmic and signaling complexes that highlight potential new functions for the importins complex beyond nucleocytoplasmic transport. Data are available via ProteomeXchange with identifier PXD032728.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Karyopherins / Antibodies, Monoclonal Type of study: Prognostic_studies Limits: Humans Language: En Journal: Mol Cell Proteomics Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2022 Type: Article Affiliation country: Israel
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Karyopherins / Antibodies, Monoclonal Type of study: Prognostic_studies Limits: Humans Language: En Journal: Mol Cell Proteomics Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2022 Type: Article Affiliation country: Israel