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Golovinomyces cichoracearum effector-associated nuclear localization of RPW8.2 amplifies its expression to boost immunity in Arabidopsis.
Zhao, Jing-Hao; Huang, Yan-Yan; Wang, He; Yang, Xue-Mei; Li, Yan; Pu, Mei; Zhou, Shi-Xin; Zhang, Ji-Wei; Zhao, Zhi-Xue; Li, Guo-Bang; Hassan, Beenish; Hu, Xiao-Hong; Chen, Xuewei; Xiao, Shunyuan; Wu, Xian-Jun; Fan, Jing; Wang, Wen-Ming.
Affiliation
  • Zhao JH; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Huang YY; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Wang H; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Yang XM; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Li Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Pu M; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Zhou SX; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Zhang JW; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Zhao ZX; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Li GB; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Hassan B; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Hu XH; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Chen X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Xiao S; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, 20850, USA.
  • Wu XJ; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Fan J; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
  • Wang WM; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China and Rice Research Institute, Sichuan Agricultural University, Chengdu, 611131, China.
New Phytol ; 238(1): 367-382, 2023 04.
Article in En | MEDLINE | ID: mdl-36522832
ABSTRACT
Arabidopsis RESISTANCE TO POWDERY MILDEW 8.2 (RPW8.2) is specifically induced by the powdery mildew (PM) fungus (Golovinomyces cichoracearum) in the infected epidermal cells to activate immunity. However, the mechanism of RPW8.2-induction is not well understood. Here, we identify a G. cichoracearum effector that interacts with RPW8.2, named Gc-RPW8.2 interacting protein 1 (GcR8IP1), by a yeast two-hybrid screen of an Arabidopsis cDNA library. GcR8IP1 is physically associated with RPW8.2 with its REALLY INTERESTING NEW GENE finger domain that is essential and sufficient for the association. GcR8IP1 was secreted and translocated into the nucleus of host cell infected with PM. Association of GcR8IP1 with RPW8.2 led to an increase in RPW8.2 in the nucleus. In turn, the nucleus-localized RPW8.2 promoted the activity of the RPW8.2 promoter, resulting in transcriptional self-amplification of RPW8.2 to boost immunity at infection sites. Additionally, ectopic expression or host-induced gene silencing of GcR8IP1 supported its role as a virulence factor in PM. Altogether, our results reveal a mechanism of RPW8.2-dependent defense strengthening via altered partitioning of RPW8.2 and transcriptional self-amplification triggered by a PM fungal effector, which exemplifies an atypical form of effector-triggered immunity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ascomycota / Arabidopsis / Arabidopsis Proteins Type of study: Prognostic_studies / Risk_factors_studies Language: En Journal: New Phytol Journal subject: BOTANICA Year: 2023 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ascomycota / Arabidopsis / Arabidopsis Proteins Type of study: Prognostic_studies / Risk_factors_studies Language: En Journal: New Phytol Journal subject: BOTANICA Year: 2023 Type: Article Affiliation country: China