Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells.

Park, Tea Soon; Hirday, Rishabh; Ali, Amir; Megersa, Roba; Villasmil, Rafael; Nguyen, Eric; Bharti, Kapil.

Affiliation

Park TS; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: teasoon.park@nih.gov.
Hirday R; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Ali A; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Megersa R; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Villasmil R; Flow Cytometry Core, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Nguyen E; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Bharti K; Ocular and Stem Cell Translational Research (OSCTR) Section, Ophthalmic Genetics and Visual Function Branch (OGVFB), National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: kapil.bharti@nih.gov.

STAR Protoc ; 4(2): 102292, 2023 May 06.

Article in En | MEDLINE | ID: mdl-37149860