Visualization of Lysosomal Dynamics during Autophagy by Fluorescent Probe.
Anal Chem
; 95(42): 15795-15802, 2023 10 24.
Article
in En
| MEDLINE
| ID: mdl-37815496
ABSTRACT
Lysosomes are one of the important organelles within cells, and their dynamic movement processes are associated with many biological events. Therefore, real-time monitoring of lysosomal dynamics processes has far-reaching implications. A lysosome-targeted fluorescent probe N(CH2)3-BD-PZ is proposed for real-time monitoring of lysosomal kinetic motility. Using this probe, the dynamic process of lysosomes under starvation induction was successfully explored through fluorescence imaging. Importantly, we observed a new pattern of lysosomal dynamic movement, in which an irregular lysosome was slowly cleaved into two different-sized touching lysosomes and then fused to form a new round lysosome. This research provides a powerful fluorescence tool to understand the dynamic motility of intracellular lysosomes under fluorescence imaging.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fluorescent Dyes
/
Lysosomes
Limits:
Humans
Language:
En
Journal:
Anal Chem
Year:
2023
Type:
Article