Klebsiella pneumoniae carbapenemase variant 44 acquires ceftazidime-avibactam resistance by altering the conformation of active-site loops.

Sun, Zhizeng; Lin, Hanfeng; Hu, Liya; Neetu, Neetu; Sankaran, Banumathi; Wang, Jin; Prasad, B V Venkataram; Palzkill, Timothy

Sun, Zhizeng; Lin, Hanfeng; Hu, Liya; Neetu, Neetu; Sankaran, Banumathi; Wang, Jin; Prasad, B V Venkataram; Palzkill, Timothy.

Affiliation

Sun Z; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Lin H; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Hu L; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Neetu N; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Sankaran B; Department of Molecular Biophysics and Integrated Bioimaging, Berkeley Center for Structural Biology, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Wang J; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Prasad BVV; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
Palzkill T; Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas, USA. Electronic address: timothyp@bcm.edu.

J Biol Chem ; 300(1): 105493, 2024 Jan.

Article in En | MEDLINE | ID: mdl-38000656

Subject(s)

Ceftazidime; Drug Resistance, Bacterial; Models, Molecular; Humans; Amino Acids/genetics; Anti-Bacterial Agents/pharmacology; Anti-Bacterial Agents/therapeutic use; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; beta-Lactamases/chemistry; beta-Lactamases/genetics; beta-Lactamases/metabolism; Ceftazidime/pharmacology; Klebsiella Infections/drug therapy; Klebsiella Infections/microbiology; Klebsiella pneumoniae/drug effects; Klebsiella pneumoniae/genetics; Drug Resistance, Bacterial/genetics; Crystallography, X-Ray; Catalytic Domain/genetics; Protein Structure, Tertiary

Key words

antibiotic resistance; antibiotics; bacteria; crystal structure; enzyme catalysis; enzyme inhibitor; enzyme kinetics; molecular dynamics; molecular evolution

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Models, Molecular / Ceftazidime / Drug Resistance, Bacterial Limits: Humans Language: En Journal: J Biol Chem Year: 2024 Type: Article Affiliation country: United States

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