A multicenter evaluation of the QIAstat-Dx meningitis-encephalitis syndromic test kit as compared to the conventional diagnostic microbiology workflow.
Eur J Clin Microbiol Infect Dis
; 43(3): 511-516, 2024 Mar.
Article
in En
| MEDLINE
| ID: mdl-38206519
ABSTRACT
PURPOSE:
Rapid diagnosis and treatment of infectious meningitis and encephalitis (ME) is critical to minimize morbidity and mortality. Recently, Qiagen introduced the CE-IVD QIAstat-Dx ME panel (QS-ME) for syndromic diagnostic testing of meningitis and encephalitis. Some data on the performance of the QS-ME in comparison to the BioFire FilmArray ME panel are available. In this study, the performance of the QS-ME is compared to the current diagnostic workflow in two academic medical centers in the Netherlands.METHODS:
A total of 110 cerebrospinal fluid samples were retrospectively tested with the QS-ME. The results obtained were compared to the results of laboratory-developed real-time PCR assays (LDTs), IS-pro, bacterial culture, and cryptococcal antigen (CrAg) testing. In addition, the accuracy of the QS-ME was also investigated using an external quality assessment (EQA) panel consisting of ten samples.RESULTS:
Four of the 110 samples tested failed to produce a valid QS-ME result. In the remaining 106 samples, the QS-ME detected 53/53 viral targets, 38/40 bacterial targets, and 7/13 Cryptococcus neoformans targets. The discrepant bacterial results consisted of two samples that were previously tested positive for Listeria monocytogenes (CT 35.8) and Streptococcus pneumoniae (CT 40), respectively. The QS-ME detected one additional result, consisting of a varicella-zoster virus signal (CT 35.9), in a sample in which both techniques detected Streptococcus pyogenes. Finally, 100% concordance was achieved in testing a blinded bacterial ME EQA panel.CONCLUSION:
The QS-ME is a relevant addition to the syndromic testing landscape to assist in diagnosing infectious ME.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Meningitis, Bacterial
/
Cryptococcus neoformans
/
Encephalitis
/
Infectious Encephalitis
/
Meningitis
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Eur J Clin Microbiol Infect Dis
Journal subject:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2024
Type:
Article
Affiliation country:
Netherlands