Traceless Peptide and Protein Modification via Rational Tuning of Pyridiniums.
J Am Chem Soc
; 146(4): 2624-2633, 2024 01 31.
Article
in En
| MEDLINE
| ID: mdl-38239111
ABSTRACT
Herein, we report a versatile reaction platform for tracelessly cleavable cysteine-selective peptide/protein modification. This platform offers highly tunable and predictable conjugation and cleavage by rationally estimating the electron effect on the nucleophilic halopyridiniums. Cleavable peptide stapling, antibody conjugation, enzyme masking/de-masking, and proteome labeling were achieved based on this facile pyridinium-thiol-exchange protocol.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Proteome
Language:
En
Journal:
J Am Chem Soc
Year:
2024
Type:
Article
Affiliation country:
China