Microfluidic paper-based analysis device applying black phosphorus nanosheets@MWCNTs-COOH: A portable and efficient strategy for detection of ß-Lactoglobulin in dairy products.
Food Chem
; 446: 138844, 2024 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-38422642
ABSTRACT
This study prepared a novel, portable and cost-effective microfluidic paper-based electrochemical analysis device (µ-PAD) using black phosphorus nanosheets@carboxylated multi-walled carbon nanotubes (BPNSs@MWCNTs-COOH) nanocomposites for ß-lactoglobulin (ß-LG) detection. At the appreciate ratio, the synthesized BPNSs@MWCNTs-COOH was demonstrated to not only serve as a high-quality substrate for the specific aptamer immobilization, but also improve the electron transfer capability of the sensing interface. The µ-PADs, utilizing BPNSs@MWCNTs-COOH and aptamer recognition, exhibited a wider detection range (10-1000 ng mL-1) and lower detection limit (LOD 0.12 ng mL-1) for ß-LG, and demonstrated enhanced specificity, satisfactory anti-interference ability and stability. When applied to the ß-LG determination in dairy samples, the µ-PAD yielded ß-LG concentrations highly correlated with those obtained using the HPLC method (R2 0.9982). These results emphasized the reliable performance of the developed µ-PADs in ß-LG allergen quantification, highlighting their potential as an efficient platform for the rapid screening of ß-LG allergens.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanotubes, Carbon
/
Lactoglobulins
Language:
En
Journal:
Food Chem
Year:
2024
Type:
Article
Affiliation country:
China