Vitexin enhances radiosensitivity of mouse subcutaneous xenograft glioma by affecting the miR-17-5p/miR-130b-3p/PTEN/HIF-1α pathway.
Strahlenther Onkol
; 200(6): 535-543, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38453699
ABSTRACT
PURPOSE:
Vitexin can cooperate with hyperbaric oxygen to sensitize the radiotherapy of glioma by inhibiting the hypoxia-inducible factor (HIF)-1α. However, whether vitexin has a direct radiosensitization and how it affects the HIF-1α expression remain unclear. This study investigated these issues.METHODS:
The SU3 cells-inoculated nude mice were divided into control, radiation, and vitexinâ¯+ radiation groups. The vitexinâ¯+ radiation-treated mice were intraperitoneally injected with 75â¯mg/kg vitexin daily for 21 days. On the 3rd, 10th, and 17th days during the vitexin treatment, the radiation-treated mice were locally irradiated with 10â¯Gy, respectively. In vitro, the microRNA (miR)-17-5p or miR-130b-3p mimics-transfected SU3 cells were used to examine the effects of vitexin plus radiation on expression of miR-17-5p- or miR-130b-3p-induced radioresistance-related pathway proteins. The effects of vitexin on miR-17-5p and miR-130b-3p expression in SU3 cells were also evaluated.RESULTS:
Compared with the radiation group, the tumor volume, tumor weight, and expression of HIF-1α, vascular endothelial growth factor, and glucose transporter-1/3 proteins, miR-17-5p, and miR-130b-3p in tumor tissues in the vitexinâ¯+ radiation group decreased, whereas the expression of phosphatase and tensin homolog (PTEN) protein increased. After treatment of miR-17-5p or miR-130b-3p mimics-transfected SU3 cells with vitexin plus radiation, the PTEN protein expression also increased, the HIF-1α protein expression decreased correspondingly. Moreover, vitexin decreased the miR-17-5p and miR-130b-3p expression in SU3 cells.CONCLUSION:
Vitexin can enhance the radiosensitivity of glioma, and its mechanism may partly be related to the attenuation of HIF-1α pathway after lowering the inhibitory effect of miR-17-5p and miR-130b-3p on PTEN.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation Tolerance
/
MicroRNAs
/
Apigenin
/
PTEN Phosphohydrolase
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Glioma
/
Mice, Nude
Limits:
Animals
/
Humans
Language:
En
Journal:
Strahlenther Onkol
/
Strahlenther. Onkol
/
Strahlentherapie und Onkologie
Journal subject:
NEOPLASIAS
/
RADIOTERAPIA
Year:
2024
Type:
Article
Affiliation country:
China