Enhancing a multi-purpose artificial urine for culture and gene expression studies of uropathogenic Escherichia coli strains.
J Appl Microbiol
; 135(4)2024 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-38486355
ABSTRACT
AIMS:
The main objective of this study was to modify a recently reported multi-purpose artificial urine (MP-AU) for culture and gene expression studies of uropathogenic Escherichia coli (UPEC) strains. METHODS ANDRESULTS:
We used liquid chromatography mass spectrometry (LC-MS) to identify and adjust the metabolic profile of MP-AU closer to that of pooled human urine (PHU). Modification in this way facilitated growth of UPEC strains with growth rates similar to those obtained in PHU. Transcriptomic analysis of UPEC strains cultured in enhanced artificial urine (enhanced AU) and PHU showed that the gene expression profiles are similar, with <7% of genes differentially expressed between the two conditions.CONCLUSIONS:
Enhancing an MP-AU with metabolites identified in PHU allows the enhanced AU to be used as a substitute for the culture and in vitro gene expression studies of UPEC strains.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Urinary Tract Infections
/
Escherichia coli Proteins
/
Escherichia coli Infections
/
Uropathogenic Escherichia coli
Limits:
Humans
Language:
En
Journal:
J Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2024
Type:
Article
Affiliation country:
United kingdom