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Targeted Repair of Spinal Cord Injury Based on miRNA-124-3p-Loaded Mesoporous Silica Camouflaged by Stem Cell Membrane Modified with Rabies Virus Glycoprotein.
Fan, Xiangchuang; Shi, Lusen; Yang, Zimeng; Li, Yiwei; Zhang, Chi; Bai, Baoshuai; Chen, Lu; Yilihamu, Elzat Elham-Yilizati; Qi, Zhangyang; Li, Wenxiang; Xiao, Peng; Liu, Mingshan; Qiu, Jichuan; Yang, Fan; Ran, Ning; Shang, Yifan; Liu, Jiaxing; Zhang, Tehan; Kong, Xiaohong; Liu, Hong; Zhou, Hengxing; Feng, Shiqing.
Affiliation
  • Fan X; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Shi L; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Yang Z; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Li Y; State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
  • Zhang C; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Bai B; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Chen L; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Yilihamu EE; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Qi Z; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Li W; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Xiao P; Key Laboratory Experimental Teratology of the Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Liu M; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Qiu J; State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
  • Yang F; Key Laboratory Experimental Teratology of the Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Ran N; Advanced Medical Research Institute, Shandong University, Jinan, 250012, P. R. China.
  • Shang Y; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Liu J; The Second Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, 250033, P. R. China.
  • Zhang T; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Kong X; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Liu H; The Second Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, 250033, P. R. China.
  • Zhou H; Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Cheeloo College of Medicine, Shandong University, Jinan, 250012, P. R. China.
  • Feng S; Advanced Medical Research Institute, Shandong University, Jinan, 250012, P. R. China.
Adv Sci (Weinh) ; 11(21): e2309305, 2024 Jun.
Article in En | MEDLINE | ID: mdl-38509833
ABSTRACT
Spinal cord injury (SCI) has no effective treatment modalities. It faces a significant global therapeutical challenge, given its features of poor axon regeneration, progressive local inflammation, and inefficient systemic drug delivery due to the blood-spinal cord barrier (BSCB). To address these challenges, a new nano complex that achieves targeted drug delivery to the damaged spinal cord is proposed, which contains a mesoporous silica nanoparticle core loaded with microRNA and a cloaking layer of human umbilical cord mesenchymal stem cell membrane modified with rabies virus glycoprotein (RVG). The nano complex more readily crosses the damaged BSCB with its exosome-resembling properties, including appropriate size and a low-immunogenic cell membrane disguise and accumulates in the injury center because of RVG, where it releases abundant microRNAs to elicit axon sprouting and rehabilitate the inflammatory microenvironment. Culturing with nano complexes promotes axonal growth in neurons and M2 polarization in microglia. Furthermore, it showed that SCI mice treated with this nano complex by tail vein injection display significant improvement in axon regrowth, microenvironment regulation, and functional restoration. The efficacy and biocompatibility of the targeted delivery of microRNA by nano complexes demonstrate their immense potential as a noninvasive treatment for SCI.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies virus / Spinal Cord Injuries / Silicon Dioxide / MicroRNAs / Disease Models, Animal Limits: Animals / Humans Language: En Journal: Adv Sci (Weinh) Year: 2024 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies virus / Spinal Cord Injuries / Silicon Dioxide / MicroRNAs / Disease Models, Animal Limits: Animals / Humans Language: En Journal: Adv Sci (Weinh) Year: 2024 Type: Article