Production of non-natural 5-methylorsellinate-derived meroterpenoids in Aspergillus oryzae.
Beilstein J Org Chem
; 20: 638-644, 2024.
Article
in En
| MEDLINE
| ID: mdl-38533468
ABSTRACT
Fungal meroterpenoids are diverse structurally intriguing molecules with various biological properties. One large group within this compound class is derived from the aromatic precursor 3,5-dimethylorsellinic acid (DMOA). In this study, we constructed engineered metabolic pathways in the fungus Aspergillus oryzae to expand the molecular diversity of meroterpenoids. We employed the 5-methylorsellinic acid (5-MOA) synthase FncE and three additional biosynthetic enzymes for the formation of (6R,10'R)-epoxyfarnesyl-5-MOA methyl ester, which served as a non-native substrate for four terpene cyclases from DMOA-derived meroterpenoid pathways. As a result, we successfully generated six unnatural 5-MOA-derived meroterpenoid species, demonstrating the effectiveness of our approach in the generation of structural analogues of meroterpenoids.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Beilstein J Org Chem
Year:
2024
Type:
Article
Affiliation country:
China