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Using the DNA Integrity Number to Analyze DNA Quality in Specimens Collected from Liquid-Based Cytology after Fine-Needle Aspiration of Breast Tumors and Lesions.
Hoshino, Akiyoshi; Oana, Yoshiyasu; Ohi, Yasuyo; Maeda, Yukari; Omori, Masako; Takada, Yuki; Ikeda, Tadashi; Sotome, Keiichi; Maeda, Hinako; Yanagisawa, Takako; Takeuchi, Osamu; Kuronuma, Satoshi; Sangai, Takafumi; Shibahara, Yukiko; Murakumo, Yoshiki; Saegusa, Makoto; Kanomata, Naoki; Nagasawa, Satoi; Yamaguchi, Rin; Yoshida, Masayuki; Kozuka, Yuji; Matsumoto, Hiroshi; Tsugawa, Koichiro; Maeda, Ichiro.
Affiliation
  • Hoshino A; Department of Diagnostic Pathology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan, frog-hoshino@umin.ac.jp.
  • Oana Y; Department of Pathology, Kitasato University School of Medicine, Tokyo, Japan, frog-hoshino@umin.ac.jp.
  • Ohi Y; Department of Diagnostic Pathology, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Maeda Y; Department of Pathology, Sagara Hospital, Kagoshima City, Kagoshima, Japan.
  • Omori M; Department of Pathology, Sagara Hospital, Kagoshima City, Kagoshima, Japan.
  • Takada Y; Department of Pathology, Kurashiki Medical Center, Kurashiki City, Okayama, Japan.
  • Ikeda T; Department of Pathology, Kurashiki Medical Center, Kurashiki City, Okayama, Japan.
  • Sotome K; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Maeda H; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Yanagisawa T; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Takeuchi O; Department of Surgery, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Kuronuma S; Biomedical Laboratory, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Sangai T; Biomedical Laboratory, Kitasato University Kitasato Institute Hospital, Tokyo, Japan.
  • Shibahara Y; Department of Surgery, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan.
  • Murakumo Y; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan.
  • Saegusa M; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan.
  • Kanomata N; Department of Pathology, Kitasato University Hospital, Sagamihara City, Sagamihara, Japan.
  • Nagasawa S; Department of Pathology, St. Lukes International Hospital, Tokyo, Japan.
  • Yamaguchi R; Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa City, Chiba, Japan.
  • Yoshida M; Department of Pathology, Nagasaki University Hospital, Nagasaki City, Nagasaki, Japan.
  • Kozuka Y; Department of Diagnostic Pathology, National Cancer Center Hospital, Tokyo, Japan.
  • Matsumoto H; Department of Pathology, Mie University Hospital, Tsu, Japan.
  • Tsugawa K; Division of Breast Surgery, Saitama Cancer Center, Saitama, Japan.
  • Maeda I; Divison of Breast and Endocrine Surgery, Department of Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
Acta Cytol ; 68(2): 145-152, 2024.
Article in En | MEDLINE | ID: mdl-38555634
ABSTRACT

INTRODUCTION:

Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage.

METHODS:

We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis.

RESULTS:

Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens.

CONCLUSION:

Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Breast Neoplasms Limits: Female / Humans / Middle aged Language: En Journal: Acta Cytol Year: 2024 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Breast Neoplasms Limits: Female / Humans / Middle aged Language: En Journal: Acta Cytol Year: 2024 Type: Article