Development of a Coumarin-derived Fluorescent Probe for Detection of HOCl and its Application in Cells and Zebrafish.
J Fluoresc
; 2024 Apr 22.
Article
in En
| MEDLINE
| ID: mdl-38647962
ABSTRACT
We have prepared a simple, universal and efficient coumarin-derived fluorescent probe (XDS1) to detecting HOCl. The experimental findings revealed that the introduction of HOCl produced an obvious quenching effect on the probe with high selectivity and sensitivity. The calculated limit of detection (LOD) was as low as 0.02 µM. Furthermore, an impressive response time of less than 10 s was observed when XDS1 detecting HOCl. Importantly, the probe XDS1 exhibited negligible cytotoxicity, thereby facilitating its application for imaging HOCl within biological environment. The probe XDS1 had been successfully used for specific detection in cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Fluoresc
Journal subject:
BIOFISICA
Year:
2024
Type:
Article