Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation.
Biochem Biophys Res Commun
; 714: 149993, 2024 Jun 25.
Article
in En
| MEDLINE
| ID: mdl-38663096
ABSTRACT
Sarcoidosis, a systemic inflammatory disease, poses challenges in understanding its etiology and variable clinical courses. Despite ongoing uncertainty about causative agents and genetic predisposition, granuloma formation remains its hallmark feature. To address this, we developed a validated in vitro human granuloma model using patient-derived peripheral blood mononuclear cells (PBMCs), offering a dynamic platform for studying early granuloma formation and sarcoidosis pathogenesis. However, a current limitation of this model is its dependence on freshly isolated PBMCs obtained from whole blood. While cryopreservation is a common method for long-term sample preservation, the biological effects of freezing and thawing PBMCs on granuloma formation remain unclear. This study aimed to assess the viability and functionality of cryopreserved sarcoidosis PBMCs within the granuloma model, revealing similar granulomatous responses to fresh cells and highlighting the potential of cryopreserved PBMCs as a valuable tool for studying sarcoidosis and related diseases.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sarcoidosis
/
Leukocytes, Mononuclear
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Cryopreservation
/
Granuloma
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2024
Type:
Article