Development of an effective single-chain variable fragment recognizing a novel epitope in the hepatitis C virus E2 protein that restricts virus entry into hepatocytes.
Arch Virol
; 169(5): 112, 2024 Apr 29.
Article
in En
| MEDLINE
| ID: mdl-38683226
ABSTRACT
Previously, we reported a neutralizing monoclonal antibody, A8A11, raised against a novel conserved epitope within the hepatitis C virus (HCV) E2 protein, that could significantly reduce HCV replication. Here, we report the nucleotide sequence of A8A11 and demonstrate the efficacy of a single-chain variable fragment (scFv) protein that mimics the antibody, inhibits the binding of an HCV virus-like particle to hepatocytes, and reduces viral RNA replication in a cell culture system. More importantly, scFv A8A11 was found to effectively restrict the increase of viral RNA levels in the serum of HCV-infected chimeric mice harbouring human hepatocytes. These results suggest a promising approach to neutralizing-antibody-based therapeutic interventions against HCV infection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Envelope Proteins
/
Hepacivirus
/
Hepatocytes
/
Virus Internalization
/
Single-Chain Antibodies
/
Epitopes
Limits:
Animals
/
Humans
Language:
En
Journal:
Arch Virol
Year:
2024
Type:
Article
Affiliation country:
India