Identifying potential breath biomarkers for early diagnosis of papillary thyroid cancer based on solid-phase microextraction gas chromatography-high resolution mass spectrometry with metabolomics.
Metabolomics
; 20(3): 59, 2024 May 21.
Article
in En
| MEDLINE
| ID: mdl-38773019
ABSTRACT
INTRODUCTION:
Thyroid cancer incidence rate has increased substantially worldwide in recent years. Fine needle aspiration biopsy (FNAB) is currently the golden standard of thyroid cancer diagnosis, which however, is invasive and costly. In contrast, breath analysis is a non-invasive, safe and simple sampling method combined with a promising metabolomics approach, which is suitable for early cancer diagnosis in high volume population.OBJECTIVES:
This study aims to achieve a more comprehensive and definitive exhaled breath metabolism profile in papillary thyroid cancer patients (PTCs).METHODS:
We studied both end-tidal and mixed expiratory breath, solid-phase microextraction gas chromatography coupled with high resolution mass spectrometry (SPME-GC-HRMS) was used to analyze the breath samples. Multivariate combined univariate analysis was applied to identify potential breath biomarkers.RESULTS:
The biomarkers identified in end-tidal and mixed expiratory breath mainly included alkanes, olefins, enols, enones, esters, aromatic compounds, and fluorine and chlorine containing organic compounds. The area under the curve (AUC) values of combined biomarkers were 0.974 (sensitivity 96.1%, specificity 90.2%) and 0.909 (sensitivity 98.0%, specificity 74.5%), respectively, for the end-tidal and mixed expiratory breath, indicating of reliability of the sampling and analysis methodCONCLUSION:
This work not only successfully established a standard metabolomic approach for early diagnosis of PTC, but also revealed the necessity of using both the two breath types for comprehensive analysis of the biomarkers.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breath Tests
/
Thyroid Neoplasms
/
Biomarkers, Tumor
/
Solid Phase Microextraction
/
Metabolomics
/
Thyroid Cancer, Papillary
/
Gas Chromatography-Mass Spectrometry
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Metabolomics
Year:
2024
Type:
Article
Affiliation country:
China