A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide in vitro and in diabetic mice.
Anal Methods
; 16(24): 3831-3838, 2024 Jun 20.
Article
in En
| MEDLINE
| ID: mdl-38828794
ABSTRACT
We designed and prepared probe W-1 for the detection of H2O2. W-1 showed excellent selectivity for H2O2 and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and H2O2 concentration (0-100 µM) provided favorable conditions for its quantitative detection. In addition, the combination of portable test strips with a smartphone platform provided great convenience for on-site visual detection of H2O2. Moreover, W-1 possessed targeting mitochondria property and could be applied to image the exogenous and endogenous H2O2 in cells to distinguish normal cells and cancer cells. Lastly, W-1 was used for monitoring the H2O2 fluctuation of the diabetic process in mice, and the results showed an increase in H2O2 levels in diabetes. Therefore, the probe provided a tool for understanding the pathological and physiological mechanisms of diabetes by imaging H2O2.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diabetes Mellitus, Experimental
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Fluorescent Dyes
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Hydrogen Peroxide
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Mitochondria
Limits:
Animals
/
Humans
Language:
En
Journal:
Anal Methods
Year:
2024
Type:
Article