An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host.
BMC Plant Biol
; 24(1): 508, 2024 Jun 06.
Article
in En
| MEDLINE
| ID: mdl-38844843
ABSTRACT
Phytophthora cinnamomi Rands is a highly prevalent phytopathogen worldwide, ranking among the top ten in terms of distribution. It inflicts crown rot, canker, and root rot on numerous plant species, significantly impacting the biodiversity of both flora and fauna within affected environments. With a host range spanning over 5,000 species, including important plants like Quercus suber, Quercus ilex, Castanea sativa, and commercially significant crops such as avocado (Persea americana), maize (Zea mays), and tomato (Solanum lycopersicum), Phytophthora cinnamomi poses a substantial threat to agriculture and ecosystems. The efficient dissemination of the oomycete relies on its short-lived asexually motile zoospores, which depend on water currents to infect host roots. However, managing these zoospores in the laboratory has long been challenging due to the complexity of the life cycle. Current protocols involve intricate procedures, including alternating cycles of growth, drought, and flooding. Unfortunately, these artificial conditions often result in a rapid decline in virulence, necessitating additional steps to maintain infectivity during cultivation. In our research, we sought to address this challenge by investigating zoospore survival under various conditions. Our goal was to develop a stable stock of zoospores that is both easily deployable and highly infective. Through direct freezing in liquid nitrogen, we have successfully preserved their virulence. This breakthrough eliminates the need for repeated culture transfers, simplifying the process of plant inoculation. Moreover, it enables more comprehensive studies of Phytophthora cinnamomi and its interactions with host plants.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phytophthora
/
Plant Diseases
Language:
En
Journal:
BMC Plant Biol
/
BMC plant biol. (Online)
/
BMC plant biology (Online)
Journal subject:
BOTANICA
Year:
2024
Type:
Article
Affiliation country:
Spain