Analysis of Raffinose Metabolism-Related Sugar Components in Seeds.
Methods Mol Biol
; 2830: 73-80, 2024.
Article
in En
| MEDLINE
| ID: mdl-38977569
ABSTRACT
Raffinose family oligosaccharides (RFOs) are synthesized from sucrose and subsequent addition of galactose moieties which was provided by galactinol. Galactinol is synthesized from UDP-galactose and myo-inositol. RFOs accumulate at late stage of seed development and play important roles in seed longevity. RFOs are major components in seeds of many plant species. Here, we document a methodology for extraction and quantitative analysis of raffinose metabolism-related soluble sugars or the derivative alcohols in plant seeds. This protocol, based on high-performance liquid chromatography (HPLC), achieves the efficient separation and accurate quantification of sucrose, myo-inositol, galactinol, and raffinose within 25 min of retention time.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Raffinose
/
Seeds
/
Sucrose
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Type:
Article
Affiliation country:
China