Photosealed Neurorrhaphy Using Autologous Tissue.
Int J Mol Sci
; 25(13)2024 Jun 25.
Article
in En
| MEDLINE
| ID: mdl-39000065
ABSTRACT
Photochemical sealing of a nerve wrap over the repair site isolates and optimizes the regenerating nerve microenvironment. To facilitate clinical adoption of the technology, we investigated photosealed autologous tissue in a rodent sciatic nerve transection and repair model. Rats underwent transection of the sciatic nerve with repair performed in three groups standard microsurgical neurorrhaphy (SN) and photochemical sealing with a crosslinked human amnion (xHAM) or autologous vein. Functional recovery was assessed at four-week intervals using footprint analysis. Gastrocnemius muscle mass preservation, histology, and nerve histomorphometry were evaluated at 120 days. Nerves treated with a PTB-sealed autologous vein improved functional recovery at 120 days although the comparison between groups was not significantly different (SN -58.4 +/- 10.9; XHAM -57.9 +/- 8.7; Vein -52.4 +/- 17.1). Good muscle mass preservation was observed in all groups, with no statistical differences between groups (SN 69 +/- 7%; XHAM 70 +/- 7%; Vein 70 +/- 7%). Histomorphometry showed good axonal regeneration in all repair techniques. These results demonstrate that peripheral nerve repair using photosealed autologous veins produced regeneration at least equivalent to current gold-standard microsurgery. The use of autologous veins removes costs and foreign body concerns and would be readily available during surgery. This study illustrates a new repair method that could restore normal endoneurial homeostasis with minimal trauma following severe nerve injury.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sciatic Nerve
/
Nerve Regeneration
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Int J Mol Sci
Year:
2024
Type:
Article
Affiliation country:
United States