The 3D organ-on-a-chip model unveils a dual role of GDF-15 in vascular growth.
Biochem Biophys Res Commun
; 733: 150441, 2024 Nov 12.
Article
in En
| MEDLINE
| ID: mdl-39059135
ABSTRACT
Pathological conditions such as oxidative stress or inflammation may alter the homeostasis of adventitia triggering vascular wall remodeling and abnormal angiogenesis, what can lead to development of atherosclerosis. Growth differentiation factor-15 (GDF-15) is a stress-responsive cytokine and metabolic regulator, but its role in angiogenesis is not yet fully defined. Here we utilized an organ-on-a-chip technology to analyze endothelial sprouting in an adventitia-resembling microenvironment. We analyzed angiogenic responses to growth factor gradient across the extracellular matrix-resembling fibrin gel and in cell co-culture in response to GDF-15-treated adventitial fibroblasts. We observed that GDF-15 enhanced the pro-angiogenic effect of vascular endothelial growth factor. On the other hand, GDF-15-treated adventitial fibroblasts decreased endothelial sprouting. GDF-15 seems to indirectly affect endothelial cells and, depending on the microenvironment, its effect can be either pro- or anti-angiogenic.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neovascularization, Physiologic
/
Growth Differentiation Factor 15
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Lab-On-A-Chip Devices
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Human Umbilical Vein Endothelial Cells
Limits:
Animals
/
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2024
Type:
Article
Affiliation country:
Poland