Altered biochemical properties of actin in normal skin fibroblasts from individuals predisposed to dominantly inherited cancers.
Cancer Res
; 46(4 Pt 1): 1867-73, 1986 Apr.
Article
in En
| MEDLINE
| ID: mdl-3948169
ABSTRACT
The data presented here show that normal skin fibroblasts from individuals with dominantly inherited retinoblastoma, polyposis coli, and nevoid basal cell carcinoma (predisposed cells), grown in the presence of [35S]methionine, contain more than 2.5-fold [35S]methionine-labeled actin as compared to normal fibroblasts from individuals without a prior history of predisposition to cancer (normal cells). The rate of incorporation of [35S]methionine into actin in predisposed cells is rapid and is not correlated with an increase in the total protein and actin contents of the cells, in the intracellular pool size of [35S]methionine, or in the synthesis of beta-actin-specific mRNA, as compared to normal cells. However, the half-life of actin in predisposed cells is less than 5 h, as compared to at least 48 h for normal cells. The significantly reduced half-life of actin and an increased incorporation of [35S]methionine specifically into actin in all predisposed cells studied may represent an inherited biochemical defect which leads to cytoskeletal disorganization previously observed in these cells. It can be speculated that the altered properties of actin in predisposed cells may be caused by the same genetic lesion(s) which is responsible for the induction of dominantly inherited cancers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actins
/
Neoplasms
Type of study:
Etiology_studies
Limits:
Humans
Language:
En
Journal:
Cancer Res
Year:
1986
Type:
Article